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KMID : 0362420050430030393
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Journal of Korean Academy of Prosthodontics
2005 Volume.43 No. 3 p.393 ~ p.408
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Gene-expression profiling of titanium-cell interaction
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Kim Chang-Su
Shin Sang-Wan
Ryu Jae-Jun
Hwang Jung-Won
Sohn Sung-Hwa
Kim Ki-Nam
Kim Meyoung-Kon
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Abstract
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Statement of problem: In the process of bone formation, titanium (Ti) surface roughness is an important factor modulating osteoblastic function.
Purpose: This study was carried out to determine the effect of different Ti surface on biologic responses of a human osteoblast-like cell line (MG63).
Materials & Methods: MG63 cells were cultured on S (smooth), SLA (sandblasted largegrit and acid etching), HA (hydroxyapatite) Ti. The morphology and attachment of the cells were examined by SEM. The cDNAs prepared from total RNAs of MG63 were hybridized to a human cDNA microarray (1,152 elements).
Results: The appearances of the surfaces observed with SEM were different in the three types of dental substrates. The surface of SLA and HA were shown to be rougher than S. MG63 cells cultured on SLA and HA were cell-matrix interaction. In the expression of genes involved in osseointegration, upregulated genes were bone morphogenetic protein, Villin, Integrin, Insulin-like growth factors in different surfaces. Downregulated genes were fibroblast growth factor receptor 4, Bcl 2-related protein, collagen, CD4 in different surfaces.
Conclusion: The attachment and expression of key osteogenic regulatory genes were enhanced by surface roughness of the dental materials.
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KEYWORD
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Titanium surfaces, expression profiling, cDNA microarray, MG63
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